{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1308888000},"Data_type":{"Still_image":false,"Z_stack":true,"Video":false,"Time_series":true},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"30563.tif","Size":173700000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"30563.jpg","Size":69842,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"30563.zip","Size":173637705,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Label":"jcb-dataviewer","Href":"http:\/\/jcb-dataviewer.rupress.org\/jcb\/browse\/1802\/"}],"PUBLISHED":["J Cell Biol. 2010 Jan 11;188(1):49-68."],"Contributors":["Gul Civelekoglu-Scholey","Li Tao","Ingrid Brust-Mascher","Roy Wollman","Jonathan M. Scholey"],"PUBMED":["PMID:20065089"]},"BIOLOGICALPROCESS":[{"onto_name":"regulation of mitotic prometaphase","onto_id":"GO:0035415"},{"onto_name":"regulation of microtubule-based process","onto_id":"GO:0032886"}],"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"A confocal 4D-stack from a transgenic Drosophila embryo expressing lamin-GFP (green) and injected with rhodamine-conjugated tubulin (red) during prometaphase shows normal elongation of the spindle during the transition during prometaphase II. This image is original data contributing to Fig. 6 \"Functional perturbation of the lamin-B envelope interferes with spindle length changes and the completion of mitosis\" from Civelekoglu-Scholey et al.(2010) Prometaphase spindle maintenance by an antagonistic motor-dependent force balance made robust by a disassembling lamin-B envelope, J. Cell Biol. 188(1):49-68."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"spindle microtubule","onto_id":"GO:0005876"},{"onto_name":"kinesin complex","onto_id":"GO:0005871"},{"onto_name":"nuclear lamina","onto_id":"GO:0005652"}],"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"8578","TECHNICALDETAILS":{"free_text":"Embryos expressing lamin-B-GFP were collected at 25°C for 1 h, matured for 40 min, dechorionated, placed on heptane glue and covered with halocarbon oil.  For injections, embryos were dehydrated for 3-6 min, covered with halocarbon oil and injected (as described in Brust-Mascher & Scholey, 2009).  Images from this image group were acquired with an inverted IX-70 Olympus with Ultra-View spinning disk confocal head (PerkinElmer) and acquired with an oil immersion objective (UPlan-Apochromat 100x N.A. 1.35, or Plan-Apochromat 60x NA 1.4).  Time series (at intervals of 3 to 10 sec) z-stacks of planes at 0.5 µm were acquired with an Orca II CCD camera (Hamamatsu Photonics).  For further information see J. Cell Biol. 188(1):49-68."},"DATAQUALIFICATION":{"free_text":"RAW"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc"},"IMAGINGMODE":{"onto_name":"spinning disk confocal microscopy","onto_id":"FBbi:00000253"},"DIMENSION":[{"Space":{"Image_size":672,"axis":"X"}},{"Space":{"Image_size":512,"axis":"Y"}},{"Space":{"Image_size":9,"Pixel_size":{"unit":"microns","value":0.5},"axis":"Z"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Drosophila melanogaster","onto_id":"NCBITaxon:7227"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":[{"onto_name":"Green fluorescent proteins from Aequorea","onto_id":"FBbi:00000437"},{"onto_name":"Rhodamine","onto_id":"FBbi:00000452"}],"CELLTYPE":{"onto_name":"early embryonic cell","onto_id":"CL:0000007"}}},"Citation":{"Title":"Gul Civelekoglu-Scholey, Li Tao, Ingrid Brust-Mascher, Roy Wollman, Jonathan M. Scholey (2011) CIL:30563, Drosophila melanogaster, early embryonic cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil30563","DOI":"doi:10.7295\/W9CIL30563"}}}