{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1304481600},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"13758.zip","Size":12599773,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"13758.tif","Size":12600000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"13758.jpg","Size":1044288,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Href":"http:\/\/jcb-dataviewer.rupress.org\/jcb\/browse\/3558\/"}],"PUBLISHED":["J Cell Biol. 2010. 191: 1173-1187."],"Contributors":["Cédric S. Asensio","Daniel W. Sirkis","Robert H. Edwards"],"PUBMED":["21149569"]},"BIOLOGICALPROCESS":{"onto_name":"regulated secretory pathway","onto_id":"GO:0045055"},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":{"onto_name":"PC-12","onto_id":"MCC:0000379"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"Electron micrograph of PC12 cells transfected with control siRNA. PC12 cells were plated onto aclar film discs (Pella) coated with poly-L-lysine, transfected twice with 50 nM control siRNA and fixed with 2.5% glutaraldehyde in calcium-\/magnesium-free PBS. The discs were washed three times with 0.1 M sodium cacodylate buffer, pH 7.2, and postfixed for 30 min on ice with 1% osmium tetroxide in cacodylate buffer containing 1.6% potassium ferricyanide. The discs were then washed three times with cacodylate buffer, three times with water, incubated with 0.5% uranyl acetate for 30 min (in the dark), and washed again with water. The samples were dehydrated in a graded series of ethanols while progressively lowering the temperature from 4°C to −40°C then embedded in epon resin. After peeling off the aclar, 60-nm sections were cut and viewed in an electron microscope (FEI Tecnai 12; Phillips) at 120 kV, images were captured with a digital camera at 6,800 magnification. Image is Fig 6C left panel and is control for Fig 6C right panel (CIL# 13764) of J Cell Biol. 2010. 191: 1173-1187."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"transport vesicle","onto_id":"GO:0030133"},{"onto_name":"trans-Golgi network transport vesicle","onto_id":"GO:0030140"},{"onto_name":"plasma membrane","onto_id":"GO:0005886"},{"onto_name":"mitochondrion","onto_id":"GO:0005739"}],"RELATIONTOINTACTCELL":{"onto_name":"cryostat-sectioned tissue","onto_id":"FBbi:00000027"},"SOURCEOFCONTRAST":{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"},"GROUP_ID":"7925","DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"}],"DIMENSION":[{"Space":{"Image_size":2048,"Pixel_size":{"unit":"microns","value":352.7778},"axis":"X"}},{"Space":{"Image_size":2048,"Pixel_size":{"unit":"microns","value":352.7778},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Rattus norvegicus","onto_id":"NCBITaxon:10116"},"PREPARATION":[{"onto_name":"tissue in epoxy resin embedment","onto_id":"FBbi:00000018"},{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"}],"VISUALIZATIONMETHODS":[{"onto_name":"osmium tetroxide","onto_id":"FBbi:00000571"},{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"}],"CELLTYPE":[{"free_text":"pheochromocytoma"},{"free_text":"adrenal gland cell"}]}},"Citation":{"Title":"Cédric S. Asensio, Daniel W. Sirkis, Robert H. Edwards (2011) CIL:13758, Rattus norvegicus, pheochromocytoma, adrenal gland cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil13758","DOI":"doi:10.7295\/W9CIL13758"}}}