{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1294203600},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"11043.jpg","Size":522364,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"11043.tif","Size":1200000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"11043.zip","Size":1394082,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"OTHER":["B. R. Beatty"],"URLs":[{"Label":"Fawcett's \"The Cell, 2nd Ed.\"","Href":"http:\/\/www.ascb.org\/bioeducate\/FawcettTheCell.htm"},{"Label":"Science Magazine","Href":"http:\/\/www.sciencemag.org\/content\/164\/3882\/955.abstract"}],"PUBLISHED":["ISBN 0721635849","from: Don W. Fawcett M.D  \u2018The Cell\u2019 2nd edition, W.B. Saunders  Chapter 4 (Nucleus)"],"Contributors":["Oscar L. Miller","Don W Fawcett"],"PUBMED":["5813982"]},"BIOLOGICALPROCESS":[{"onto_name":"nucleus organization","onto_id":"GO:0006997"},{"onto_name":"RNA metabolic process","onto_id":"GO:0016070"}],"PROCESSINGHISTORY":{"free_text":"Image extracted from .pdf file of 'The Cell'"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"Transmission electron micrograph of transcription of tandemly arranged ribosomal RNA genes. Amphibian oocyte nuclei contain numerous extra-chromosomal nucleoli, each of which contains a tandem array of genes encoding ribosomal RNA. Here, using the methods developed by O.L. Miller Jr. and colleagues, the extrachromosomal nucleoli from a newt oocyte have been dispersed in low salt, and centrifuged through a sucrose cushion containing formaldehyde on to a carbon coated grid and stained for TEM imaging. Active transcription is seen by the tree-like structures in which each branch represents a growing transcript.  Untranscribed spacer DNA is seen between each transcript."},"ITEMTYPE":{"onto_name":"film","onto_id":"FBbi:00000303"},"CELLULARCOMPONENT":[{"onto_name":"nucleolar chromatin","onto_id":"GO:0030874"},{"onto_name":"chromatin","onto_id":"GO:0000785"},{"free_text":"ribosomal RNA"}],"RELATIONTOINTACTCELL":{"onto_name":"isolated subcellular component","onto_id":"FBbi:00000579"},"SOURCEOFCONTRAST":{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"},"MOLECULARFUNCTION":{"free_text":"rRNA transcription"},"TECHNICALDETAILS":{"free_text":"Micrograph originally published in Miller & Beatty, Science 164:955-957, and reprinted with permission as Figure 144 from Chapter 4 (Nucleus) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D.  A PDF copy of the accompanying chapter is available on the ASCB's BioEDUCATE website."},"DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_nd"},"IMAGINGMODE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},{"onto_name":"detection of electrons","onto_id":"FBbi:00000375"}],"DIMENSION":[{"Space":{"Image_size":920,"axis":"X"}},{"Space":{"Image_size":1296,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Notophthalmus viridescens","onto_id":"NCBITaxon:8316"},"PREPARATION":{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},"VISUALIZATIONMETHODS":{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},"CELLTYPE":{"onto_name":"oocyte","onto_id":"CL:0000023"}}},"Citation":{"Title":"Oscar L. Miller, Don W Fawcett (2011) CIL:11043, Notophthalmus viridescens, oocyte. CIL. Dataset","ARK":"ark:\/b7295\/w9cil11043","DOI":"doi:10.7295\/W9CIL11043"}}}