CIL:11043, Notophthalmus viridescens, oocyte
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- Cite This Work
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Miller, Oscar L.; Fawcett, Don W. (2021). CIL:11043, Notophthalmus viridescens, oocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0TQ60BW
- Description
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Transmission electron micrograph of transcription of tandemly arranged ribosomal RNA genes. Amphibian oocyte nuclei contain numerous extra-chromosomal nucleoli, each of which contains a tandem array of genes encoding ribosomal RNA. Here, using the methods developed by O.L. Miller Jr. and colleagues, the extrachromosomal nucleoli from a newt oocyte have been dispersed in low salt, and centrifuged through a sucrose cushion containing formaldehyde on to a carbon coated grid and stained for TEM imaging. Active transcription is seen by the tree-like structures in which each branch represents a growing transcript. Untranscribed spacer DNA is seen between each transcript.
- Date Issued
- 2021
- Researchers
- Methods
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Micrograph originally published in Miller & Beatty, Science 164:955-957, and reprinted with permission as Figure 144 from Chapter 4 (Nucleus) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. A PDF copy of the accompanying chapter is available on the ASCB's BioEDUCATE website.
- Technical Details
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Preparation: formaldehyde fixed tissue
Relation to intact cell: isolated subcellular component
Item type: film
Imaging mode: transmission electron microscopy (TEM); detection of electrons
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Processing history: Image extracted from .pdf file of 'The Cell'
Data qualification: Processed - Scientific Name
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- Identifier
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Samplenumber: 11043
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL11043
- Miller OL Jr, Beatty BR. Visualization of nucleolar genes. Science. 1969 May 23;164(3882):955-7. https://doi.org/10.1126/science.164.3882.955
- PubMed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=5813982
- Fawcett, Don W. "The Cell." 2nd ed., W.B. Saunders, 1981, ISBN 0721635849. https://www.ascb.org/career-development/teaching/don-fawcetts-the-cell/
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- License
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Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12