{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1311739200},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"35431.jpg","Size":62551,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"35431.zip","Size":1244262,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"35431.tif","Size":1300000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Href":"http:\/\/www.plosone.org\/article\/info:doi%2F10.1371%2Fjournal.pone.0003338"}],"Contributors":["Stephane Brunet","Julien Dumont","Karen W. Lee","Kazuhisha Kinoshita","Pascale Hikal","Oliver J. Gruss","Bernard Maro","Marie-Helene Verlhac"],"PUBMED":["18833336"]},"BIOLOGICALPROCESS":[{"onto_name":"meiotic spindle organization","onto_id":"GO:0000212"},{"onto_name":"microtubule organizing center organization","onto_id":"GO:0031023"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"This 3 panel image displays meiosis in a mouse oocyte triple labelled for  microtubules (panel 1 and green), phosphorylated-Transforming Acidic Coiled-Coil protein (TACC3; panel 2 and red), and nucleic acids (blue). This oocyte was injected with siTPX2-5h, a doublestranded siRNA against TPX2 (targeting protein for Xenopus kinesin-like protein, xklp2)and rescued with the coinjection of YFP-TPX2.\n\nFigure 7a, third row. doi:10.1371\/journal.pone.0003338.g007"},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"microtubule","onto_id":"GO:0005874"},{"onto_name":"microtubule organizing center part","onto_id":"GO:0044450"},{"onto_name":"nuclear chromosome","onto_id":"GO:0000228"}],"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"distribution of epitope","onto_id":"FBbi:00000592"},"GROUP_ID":"8819","MOLECULARFUNCTION":{"onto_name":"GTPase activity","onto_id":"GO:0003924"},"TECHNICALDETAILS":{"free_text":"Time-lapse images were acquired using a Photometrics CCD camera (CoolSnap HQ2) mounted on a Leica DMI600B microscope using a Leica HC PL APO 20X\/0.7 NA objective enclosed in a thermostatic chamber (Life Imaging Services). Immunofluorescence methods can be found in the cited paper and in Brunet S. et al. (1999) J Cell Biol 146: 1\u201311."},"DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"attribution_cc_by"},"IMAGINGMODE":{"onto_name":"confocal microscopy","onto_id":"FBbi:00000251"},"DIMENSION":[{"Space":{"Image_size":1310,"Pixel_size":{"unit":"microns","value":0.1},"axis":"X"}},{"Space":{"Image_size":310,"Pixel_size":{"unit":"microns","value":0.1},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"},"PREPARATION":{"onto_name":"chemically fixed tissue","onto_id":"FBbi:00000002"},"VISUALIZATIONMETHODS":{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"},"CELLTYPE":{"onto_name":"oocyte","onto_id":"CL:0000023"}}},"Citation":{"Title":"Stephane Brunet, Julien Dumont, Karen W. Lee, Kazuhisha Kinoshita, Pascale Hikal, Oliver J. Gruss, Bernard Maro, Marie-Helene Verlhac (2011) CIL:35431, Mus musculus, oocyte. CIL. Dataset","ARK":"ark:\/b7295\/w9cil35431","DOI":"doi:10.7295\/W9CIL35431"}}}