{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1320379200},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"38706.zip","Size":2375808,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"38706.tif","Size":2400000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"38706.jpg","Size":618348,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Label":"Journal of Cell Biology","Href":"http:\/\/jcb.rupress.org\/content\/48\/3\/594.full.pdf+html?sid=6c2cff91-8475-4246-8ec9-caaf88a6a9f5"}],"PUBLISHED":["UW Goodenough and LA Staehelin (1971) Structural differentiation of stacked and unstacked chloroplast membranes. J Cell Biol 48:595-619."],"Contributors":["Ursula W. Goodenough","L. Andrew Staehelin"],"PUBMED":["4396088"]},"BIOLOGICALPROCESS":[{"onto_name":"photosynthesis","onto_id":"GO:0015979"},{"onto_name":"organelle organization","onto_id":"GO:0006996"}],"CELLLINE":[{"free_text":"ac-5"},{"free_text":"ac-31"}],"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"Isolated chloroplast membranes of the unicellular photosynthetic alga Chlamydomonas reinhardtii were exposed to low salt buffer, cryofixed, freeze-fractured, and surface replicas observed by transmission electron microscopy. Under these conditions, thylakoid membranes from wildtype cells show the particle size and distribution typical of unstacked membranes. See Fig 22 in Goodenough and Staehelin 1971 J Cell Biol 48:595-619."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"chloroplast","onto_id":"GO:0009507"},{"onto_name":"plastid","onto_id":"GO:0009536"},{"onto_name":"plastid thylakoid membrane","onto_id":"GO:0055035"}],"SOURCEOFCONTRAST":{"onto_name":"differences in deposition of metal shadow","onto_id":"FBbi:00000601"},"RELATIONTOINTACTCELL":{"onto_name":"isolated subcellular component","onto_id":"FBbi:00000579"},"GROUP_ID":"11387","TECHNICALDETAILS":{"free_text":"Isolated thylakoid membranes were cryoprotected with glycerol, cryofixed, fractured at -100C, shadowed with Pt, and replicas prepared by carbon coating. Cleaned replicas were examined with a Philips 300 TEM. See Goodenough and Staehelin 1971 J Cell Biol 48:595-619"},"DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},"DIMENSION":[{"Space":{"Image_size":1590,"axis":"X"}},{"Space":{"Image_size":1494,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Chlamydomonas reinhardtii","onto_id":"NCBITaxon:3055"},"PREPARATION":[{"onto_name":"cryofixed tissue","onto_id":"FBbi:00000013"},{"onto_name":"freeze_fracture\/freeze_etch","onto_id":"FBbi:00000419"}],"VISUALIZATIONMETHODS":{"onto_name":"shadowing and plating","onto_id":"FBbi:00000398"}}},"Citation":{"Title":"Ursula W. Goodenough, L. Andrew Staehelin (2011) CIL:38706, Chlamydomonas reinhardtii. CIL. Dataset","ARK":"ark:\/b7295\/w9cil38706","DOI":"doi:10.7295\/W9CIL38706"}}}