{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1307505600},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"13889.zip","Size":190822,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"13889.tif","Size":200000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"13889.jpg","Size":19848,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Href":"http:\/\/jcb-dataviewer.rupress.org\/jcb\/browse\/4053\/"}],"PUBLISHED":["J Cell Biol. (2011) 192: 599-614."],"Contributors":["Mauricio Valerio-Santiago","Fernando Monje-Casas"],"PUBMED":["21321099"]},"BIOLOGICALPROCESS":[{"onto_name":"regulation of exit from mitosis","onto_id":"GO:0007096"},{"onto_name":"small GTPase mediated signal transduction","onto_id":"GO:0007264"},{"onto_name":"mitosis","onto_id":"GO:0007067"},{"onto_name":"cell division","onto_id":"GO:0051301"},{"onto_name":"cell cycle","onto_id":"GO:0007049"},{"onto_name":"mitotic cell cycle spindle orientation checkpoint","onto_id":"GO:0031578"},{"onto_name":"exit from mitosis","onto_id":"GO:0010458"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":{"free_text":"W303"},"PARAMETERIMAGED":[{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},{"onto_name":"optical path length gradient","onto_id":"FBbi:00000312"}],"IMAGEDESCRIPTION":{"free_text":"Tem1 (green) constitutively targeted to spindle pole bodies in nud1-2 cells at the restrictive temperature still displayed problems positioning the spindle (this image shows a misaligned spindle, CIL #13873 shows an aligned spindle and CIL# 13874 shows a rebudded cell) typical of nud1-2, despite the fact that viability was restored. Nud1 is required for mitotic exit and constitutive targeting of Tem1 recovered the mitotic exit defect of nud1-2 cells. DAPI (blue) and differential interference contrast (DIC) image are also shown. Image is Fig 7F, middle panels, in J Cell Biol. (2011) 192: 599-614. Images in Fig 7 include CIL #13888, 13872, 13813, 13889, 13874."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"cytoskeleton","onto_id":"GO:0005856"},{"onto_name":"spindle pole body","onto_id":"GO:0005816"},{"onto_name":"cytoplasm","onto_id":"GO:0005737"},{"onto_name":"nucleus","onto_id":"GO:0005634"},{"onto_name":"nuclear envelope","onto_id":"GO:0005635"}],"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":[{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},{"onto_name":"boundaries between regions with different refractive index","onto_id":"FBbi:00000599"},{"onto_name":"compartmentalization of stain or label","onto_id":"FBbi:00000604"}],"GROUP_ID":"9034","MOLECULARFUNCTION":[{"onto_name":"GTPase activity","onto_id":"GO:0003924"},{"onto_name":"nucleotide binding","onto_id":"GO:0000166"},{"onto_name":"protein binding","onto_id":"GO:0005515"},{"onto_name":"GTPase activator activity","onto_id":"GO:0005096"},{"onto_name":"structural constituent of cytoskeleton","onto_id":"GO:0005200"}],"TECHNICALDETAILS":{"free_text":"Cells (MATa nud1::kanMX4 leu2::nud1-2::LEU2 pRS316::eGFP-CNM67\u2013TEM1) grown at restrictive temperature 37C were fixed in 2.5% formaldehyde for 10 min, washed twice, and resuspended in 0.1 M potassium phosphate buffer, pH 6.4. Cells were then fixed for 10 min in 80% ethanol and resuspended in 1 mg\/ml DAPI. Imaging was performed at 25C using a Leica DM6000 microscope equipped with a 100x\/1.40 NA oil immersion objective lens, A4, L5, and TX2 filters, and a digital CCD camera (DFC350, Leica). Pictures were processed with LAS AF (Leica) and ImageJ software."},"DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":[{"onto_name":"widefield illumination","onto_id":"FBbi:00000277"},{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},{"onto_name":"differential interference contrast microscopy","onto_id":"FBbi:00000245"}],"DIMENSION":[{"Space":{"Image_size":249,"Pixel_size":{"unit":"microns","value":0.0642},"axis":"X"}},{"Space":{"Image_size":249,"Pixel_size":{"unit":"microns","value":0.0642},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Saccharomyces cerevisiae","onto_id":"NCBITaxon:4932"},"PREPARATION":[{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},{"onto_name":"ethanol fixed tissue","onto_id":"FBbi:00000006"}],"VISUALIZATIONMETHODS":[{"onto_name":"EGFP","onto_id":"FBbi:00000082"},{"onto_name":"4',6-diamidino-2-phenylindole (DAPI)","onto_id":"FBbi:00000056"}]}},"Citation":{"Title":"Mauricio Valerio-Santiago, Fernando Monje-Casas (2011) CIL:13889, Saccharomyces cerevisiae. CIL. Dataset","ARK":"ark:\/b7295\/w9cil13889","DOI":"doi:10.7295\/W9CIL13889"}}}