{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1308715200},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"24479.jpg","Size":324080,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"24479.tif","Size":1200000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"Contributors":["Mark S. Ladinsky","J. Richard McIntosh","David N. Mastronarde","Kathryn E. Howell","L. Andrew Staehelin"]},"BIOLOGICALPROCESS":[{"onto_name":"Golgi organization","onto_id":"GO:0007030"},{"onto_name":"Golgi vesicle budding","onto_id":"GO:0048194"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":{"onto_name":"NRK-52E","onto_id":"MCC:0000366"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"This image is from a tomogram through part of a Golgi ribbon from a normal rat kidney cell, generated using high voltage electron microscopy following incubation at 15°C to block transport out of the Golgi complex. Following temperature block, large bulging domains appear on the three trans-most cisternae. The sample was tilted at angles of 1.5° to obtain the series of images in this set, and used to obtain 3-dimensional reconstructions of the Golgi apparatus and contributed to findings reported in Ladinsky et al. (1999) Golgi structure in three dimensions: Functional insights from the normal rat kidney cell. J. Cell Biol. 144 (6) 1135-1149."},"ITEMTYPE":{"onto_id":"FBbi:00000622"},"CELLULARCOMPONENT":[{"onto_name":"Golgi apparatus","onto_id":"GO:0005794"},{"onto_name":"Golgi cisterna","onto_id":"GO:0031985"}],"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},"GROUP_ID":"8574","TECHNICALDETAILS":{"free_text":"Cells were grown on 100-mesh gold EM grids, and maintained at 15°C for 4 hours before plunge freezing in liquid nitrogen (BalTec HPM-010), followed by freeze-substitution (1% glutaraldehyde, 0.1% tannic acid in acetone, replaced with 4% osmium tetroxide and 0.01% uranyl acetate), then embedded in Epon-Araldite and sectioned at 250nm (UltraCut-UCT, Leica). Sections were transferred to formvar-coated copper-rhodium slot grids (EMS) and stained with 2% aqueous uranyl acetate and Reynold's lead citrate. Digital images were acquired at a magnification of 15,000X with an FEI Tecnai TF20 transmission EM. For additional details refer to: J. Cell Biol. 144 (6) 1135-1149."},"DATAQUALIFICATION":{"free_text":"RAW"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":[{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"},{"onto_name":"detection of electrons","onto_id":"FBbi:00000375"}],"DIMENSION":[{"Space":{"Image_size":1078,"axis":"X"}},{"Space":{"Image_size":1031,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Rattus norvegicus","onto_id":"NCBITaxon:10116"},"PREPARATION":[{"onto_id":"FBbi:00000621"},{"onto_name":"tissue in epoxy resin embedment","onto_id":"FBbi:00000018"}],"VISUALIZATIONMETHODS":[{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"}],"CELLTYPE":{"onto_name":"epithelial cell","onto_id":"CL:0000066"}}},"Citation":{"Title":"Mark S. Ladinsky, J. Richard McIntosh, David N. Mastronarde, Kathryn E. Howell, L. Andrew Staehelin (2011) CIL:24479, Rattus norvegicus, epithelial cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil24479","DOI":"doi:10.7295\/W9CIL24479"}}}