Allen, Richard (2021). CIL:38861, Didinium nasutum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0WM1CQ2

A high resolution view of the cytopharynx but in a dividing Didinium. This cytopharynx is in the proter (anterior) daughter cell. The lamellae, each consisting of a set of perpendicularly arranged microtubules cover this food-vacuole forming region. Vesicles lie near the cytopharyngeal membrane and fuse with the membrane. TEM taken on 5/20/69 by R. Allen with Philips 300 operating at 60kV. Neg. 6,370X.

• 2021

• Allen, Richard

The raw film was scanned with a Nikon Coolscan 9000ED. This digitized image is available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 9769

• Didinium nasutum

• Cell by organism
• Cell cortex
• Ciliated Protist
• Cortical microtubule cytoskeleton
• Eukaryotic cell
• Eukaryotic Protist

• Carolina Biological Supply Company, NC, U.S.A.
• Cortical cytoskeleton organization
• Cortical microtubule organization
• Plasma membrane organization
• Structural constituent of cytoskeleton
• Structural molecule activity

• data
• image

• No linguistic content; Not applicable

Samplenumber: 38861

Doi: https://doi.org/10.6075/J0WM1CQ2

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL38861

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2022-08-12