{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1409889600},"Data_type":{"Still_image":false,"Z_stack":false,"Video":true,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"47519.jpg","Size":228160,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Mp4","File_path":"47519_web.mp4","Size":1575973,"Mime_type":"video\/mp4"},{"File_type":"Zip","File_path":"47519.zip","Size":316108928,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"Contributors":["Mark Harding"]},"BIOLOGICALPROCESS":{"free_text":"immune cell recruitment"},"PROCESSINGHISTORY":{"free_text":"Converted from volocity to .avi"},"IMAGEDESCRIPTION":{"free_text":"Visualization of neutrophil recruitment in capillaries proximal to S. aureus beads. (Figure 3 - A4 block)"},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"RELATIONTOINTACTCELL":{"free_text":"intact cells-in vivo imaging"},"GROUP_ID":"16605","MOLECULARFUNCTION":{"free_text":"blocking of integrin α4"},"TECHNICALDETAILS":{"free_text":"Mice were anesthetized with ketamine\/xylazine, and then subcutaneous tissue on a dorsal flap was exteriorized.  GFP labelled S. aureus beads were inserted.  Antibodies were injected into the vasculature to label neutrophils (red) and endothelial cells (blue)"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"spinning disk confocal microscopy","onto_id":"FBbi:00000253"},"DIMENSION":[{"Space":{"Pixel_size":{"unit":"microns","value":512},"axis":"X"}},{"Space":{"Pixel_size":{"unit":"microns","value":512},"axis":"Y"}}],"SPECIESTAXASPECIFIC":{"onto_id":":mouse (C57 Black 6)"},"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Staphylococcus aureus","onto_id":"NCBITaxon:1280"},"CELLTYPE":[{"onto_name":"neutrophil","onto_id":"CL:0000775"},{"onto_name":"endothelial cell","onto_id":"CL:0000115"}],"PATHOLOGICALPROCESS":{"free_text":":Control of Infection of S. aureus (insertion of sterile agarose beads)  Imaging Methods spinning disk confocal microscopy+dissection of mouse sub cutaneous tissue"}}},"Citation":{"Title":"Mark Harding (2014) CIL:47519, Staphylococcus aureus, neutrophil, endothelial cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil47519","DOI":"doi:10.7295\/W9CIL47519"}}}