{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1382241600},"Data_type":{"Still_image":false,"Z_stack":true,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"45451.zip","Size":209749033,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"45451.tif","Size":210000000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"45451.jpg","Size":379230,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"Contributors":["Assaf Zaritsky"]},"BIOLOGICALPROCESS":[{"free_text":"cell migration"},{"free_text":"wound healing"}],"PROCESSINGHISTORY":{"free_text":"unprocessed raw data"},"CELLLINE":{"free_text":"DA3"},"PARAMETERIMAGED":{"free_text":"optical path length gradient"},"IMAGEDESCRIPTION":{"free_text":"Wound Healing Assay Time series DIC images of mouse DA3 cells, derived from the mouse mammary adenocarcinoma cell line D1-DMBA-3, induced in BALB\/C mice by dimethylbenzanthracene were grown to 90% confluence. A scratch approximately 300um wide was made in the cell monolayer, and images were recorded every 14.5 min for 26 hr. This time series image, obtained with PHA (c-Met inhibitor) treatment, is part of a group of 4 time series images (CIL:45451 to CIL:45454)."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"SOURCEOFCONTRAST":{"free_text":"boundaries between regions with different refractive index"},"GROUP_ID":"17052","TECHNICALDETAILS":{"free_text":"DA3 cells expressing the fluorescent protein mCherry were grown to 90% confluence in wells of 2 cm diameter in DMEM supplemented with 10% heat-inactivated FCS (Gibco-BRL) and treated with the Met inhibitor PHA665752 (2.5 µM) for 2 hrs. A scratch was generated using a 200 µl tip, and the cells were incubated and subjected to time lapse confocal laser scanning microscopy (CLSM-510, Zeiss, Germany) for approximately 26 hrs, with images taken at 14.5 min intervals. The position of each scratch was predefined, and a macro that repetitively positions the microscope on each point was executed. The acquired differential interference contrast (DIC) channel of the time-lapse sequence (shown here) was used for the multi-cellular analysis."},"DATAQUALIFICATION":{"free_text":"RAW"},"TERMSANDCONDITIONS":{"free_text":"attribution_cc_by"},"IMAGINGMODE":{"free_text":"differential interference contrast microscopy"},"DIMENSION":[{"Space":{"Image_size":1024,"Pixel_size":{"unit":"microns","value":1.24},"axis":"X"}},{"Space":{"Image_size":1024,"Pixel_size":{"unit":"microns","value":1.24},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"},"PREPARATION":{"free_text":"living tissue"},"VISUALIZATIONMETHODS":{"free_text":"visualization of contiguous regions"},"CELLTYPE":{"free_text":"mammary adenocarcinoma"}}},"Citation":{"Title":"Assaf Zaritsky (2013) CIL:45451, Mus musculus, mammary adenocarcinoma. CIL. Dataset","ARK":"ark:\/b7295\/w9cil45451","DOI":"doi:10.7295\/W9CIL45451"}}}