{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1311739200},"Data_type":{"Still_image":false,"Z_stack":false,"Video":false,"Time_series":true},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"35426.tif","Size":3700000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"35426.jpg","Size":1334,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"35426.zip","Size":3605001,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Href":"http:\/\/www.plosone.org\/article\/info:doi%2F10.1371%2Fjournal.pone.0003338"}],"Contributors":["Stephane Brunet","Julien Dumont","Karen W. Lee","Kazuhisha Kinoshita","Pascale Hikal","Oliver J. Gruss","Bernard Maro","Marie-Helene Verlhac"],"PUBMED":["18833336"]},"BIOLOGICALPROCESS":[{"onto_name":"meiotic spindle organization","onto_id":"GO:0000212"},{"onto_name":"microtubule organizing center organization","onto_id":"GO:0031023"},{"onto_name":"RNA interference","onto_id":"GO:0016246"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Time-lapse microscopy of histone-RFP expressing mouse oocytes injected with siTPX2-5h, a doublestranded siRNA against TPX2, (targeting protein for Xenopus kinesin-like protein, xklp2). Images were taken every 20 minutes. Times after the resumption of meiotic maturation and germinal vesicle breakdown (GVBD) are indicated in the lower left corner.\n\nVideo S6 Found at: doi:10.1371\/journal.pone.0003338.s006"},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"microtubule","onto_id":"GO:0005874"},{"onto_name":"microtubule organizing center part","onto_id":"GO:0044450"},{"onto_name":"nuclear chromosome","onto_id":"GO:0000228"}],"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"8819","MOLECULARFUNCTION":{"onto_name":"GTPase activity","onto_id":"GO:0003924"},"TECHNICALDETAILS":{"free_text":"Time-lapse images were acquired using a Photometrics CCD camera (CoolSnap HQ2) mounted on a Leica DMI600B microscope using a Leica HC PL APO 20X\/0.7 NA objective enclosed in a thermostatic chamber (Life Imaging Services). Immunofluorescence methods can be found in the cited paper and in Brunet S. et al. (1999) J Cell Biol 146: 1\u201311."},"DATAQUALIFICATION":{"free_text":"RAW"},"TERMSANDCONDITIONS":{"free_text":"attribution_cc_by"},"IMAGINGMODE":{"onto_name":"confocal microscopy","onto_id":"FBbi:00000251"},"DIMENSION":[{"Space":{"Image_size":150,"axis":"X"}},{"Space":{"Image_size":150,"axis":"Y"}},{"Time":{"unit":"microns","value":900,"frame":"53"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"onto_name":"DsRed","onto_id":"FBbi:00000085"},"CELLTYPE":{"onto_name":"oocyte","onto_id":"CL:0000023"}}},"Citation":{"Title":"Stephane Brunet, Julien Dumont, Karen W. Lee, Kazuhisha Kinoshita, Pascale Hikal, Oliver J. Gruss, Bernard Maro, Marie-Helene Verlhac (2011) CIL:35426, Mus musculus, oocyte. CIL. Dataset","ARK":"ark:\/b7295\/w9cil35426","DOI":"doi:10.7295\/W9CIL35426"}}}