{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1306900800},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"13748.zip","Size":791949,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"13748.tif","Size":800000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"13748.jpg","Size":33576,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Href":"http:\/\/jcb-dataviewer.rupress.org\/jcb\/browse\/3512"}],"PUBLISHED":["J Cell Biol. 2010. 191: 943-952."],"Contributors":["Tiffiney R. Hartman","Daniel Zinshteyn","Heather K. Schofield","Emmanuelle Nicolas","Ami Okada","Alana M. O'Reilly"],"PUBMED":["21098113"]},"BIOLOGICALPROCESS":[{"onto_name":"germ-line stem cell division","onto_id":"GO:0042078"},{"onto_name":"ovarian follicle cell development","onto_id":"GO:0030707"},{"onto_name":"positive regulation of smoothened signaling pathway","onto_id":"GO:0045880"},{"onto_name":"positive regulation of hh target transcription factor activity","onto_id":"GO:0007228"},{"onto_name":"smoothened signaling pathway","onto_id":"GO:0007224"},{"onto_name":"cell adhesion","onto_id":"GO:0007155"},{"onto_name":"germ-line cyst formation","onto_id":"GO:0048134"},{"onto_name":"germarium-derived egg chamber formation","onto_id":"GO:0007293"},{"onto_name":"positive regulation of translation","onto_id":"GO:0045727"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":{"free_text":"boi[e]"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"In the absence of boi (boi[e] mutant), Hedgehog (Hh, red) is redistributed from apical cells to the extracellular space of the local follicle stem cell niche (CIL# 13744, 13745). Expression of Boi in apical cells relocalizes Hh to apical cells (CIL#13746). Apical cell expression of Ihog, which also binds Hh directly, rescues Hh localization in the boi[e] mutant (this image; boi[e]; ihog[EP]\/+; babGal4\/+). Germ cells are identified with anti-Vasa (blue). Image is Fig 3E in J Cell Biol. 2010. 191: 943-952. Images in Fig 3 include CIL# 13744, 13745, 13746, 13747, 13748."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"extracellular region","onto_id":"GO:0005576"},{"onto_name":"cytoplasmic membrane-bounded vesicle","onto_id":"GO:0016023"},{"onto_name":"endocytic vesicle","onto_id":"GO:0030139"},{"onto_name":"P granule","onto_id":"GO:0043186"}],"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"8382","MOLECULARFUNCTION":[{"onto_name":"protein binding","onto_id":"GO:0005515"},{"onto_name":"morphogen activity","onto_id":"GO:0016015"},{"onto_name":"patched binding","onto_id":"GO:0005113"},{"onto_name":"cysteine-type endopeptidase activity","onto_id":"GO:0004197"},{"onto_name":"mRNA 3'-UTR binding","onto_id":"GO:0003730"},{"onto_name":"RNA binding","onto_id":"GO:0003723"},{"onto_name":"ATP-dependent helicase activity","onto_id":"GO:0008026"}],"TECHNICALDETAILS":{"free_text":"Fly ovaries were dissected and fixed as described previously (O\u2019Reilly et al., 2008). Primary antibodies were 1:2,000 rabbit anti-Vasa; 1:100 goat anti-Hh (Santa Cruz Biotechnology, Inc.). Secondary antibodies used were FITC, Cy3, and\/or Cy5 conjugated to species-specific secondary antibodies (Jackson ImmunoResearch Laboratories, Inc.). Samples were mounted in Vectashield mounting medium. Images were collected at room temperature (22C) using 40× (1.25 NA) or 63× (1.4 NA) oil immersion lenses (Leica) on an upright microscope (DM 5000; Leica) coupled to a confocal laser scanner (TCS SP5; Leica). LAS AF SP5 software (Leica) was used for data acquisition. Images representing individual channels of single confocal slices from the center of each germarium were exported as TIFF files, and images were converted to figures using Photoshop software (Adobe)."},"DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"single-spot confocal microscopy","onto_id":"FBbi:00000252"},"DIMENSION":[{"Space":{"Image_size":512,"Pixel_size":{"unit":"microns","value":0.2084},"axis":"X"}},{"Space":{"Image_size":512,"Pixel_size":{"unit":"microns","value":0.2084},"axis":"Y"}},{"Space":{"Image_size":1,"Pixel_size":{"unit":"microns","value":0.042},"axis":"Z"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Drosophila melanogaster","onto_id":"NCBITaxon:7227"},"PREPARATION":[{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},{"onto_name":"detergent permeabilized","onto_id":"FBbi:00000262"}],"VISUALIZATIONMETHODS":{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"},"CELLTYPE":[{"onto_name":"germ line cell","onto_id":"CL:0000039"},{"onto_name":"follicle cell","onto_id":"CL:0000477"},{"onto_name":"germ line stem cell","onto_id":"CL:0000014"},{"onto_name":"follicle stem cell","onto_id":"CL:0000441"}]}},"Citation":{"Title":"Tiffiney R. Hartman, Daniel Zinshteyn, Heather K. Schofield, Emmanuelle Nicolas, Ami Okada, Alana M. O'Reilly (2011) CIL:13748, Drosophila melanogaster, germ line cell, follicle cell, germ line stem cell, follicle stem cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil13748","DOI":"doi:10.7295\/W9CIL13748"}}}