{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1301457600},"Data_type":{"Still_image":false,"Z_stack":true,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"13465.tif","Size":900000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"13465.jpg","Size":13575,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"13465.zip","Size":842182,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Href":"http:\/\/jcb-dataviewer.rupress.org\/jcb\/browse\/1851\/"}],"PUBLISHED":["J Cell Biol. 187: 967-975. 2009"],"Contributors":["Christopher S. Wood","Karl R. Schmitz","Nicholas J. Bessman","Thanuja Gangi Setty","Kathryn M. Ferguson","Christopher G. Burd"],"PUBMED":["20026658"]},"BIOLOGICALPROCESS":[{"onto_name":"cell wall mannoprotein biosynthetic process","onto_id":"GO:0000032"},{"onto_name":"protein glycosylation","onto_id":"GO:0006486"},{"onto_name":"phosphatidylinositol-mediated signaling","onto_id":"GO:0048015"}],"PROCESSINGHISTORY":{"onto_name":"constrained iterative deconvolution","onto_id":"FBbi:00000360"},"CELLLINE":{"free_text":"pik1-83"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Kre2-GFP is not efficiently maintained in the Golgi apparatus when Pik1-mediated PtdIns4P synthesis ceases, implicating Pik1 signaling in retrograde trafficking of Golgi residents. In this image, Kre2-GFP is expressed in the pik1-83 mutant strain after shift to restrictive temperature of 37C for 1h. Cells grown in liquid medium were mounted in growth medium and 3D image stacks were collected at 0.4-µm z increments on a DeltaVision workstation (Applied Precision) based on an inverted microscope (IX-70; Olympus) using a 100× NA 1.4 oil immersion lens. Images were captured at 23C with a 12-bit CCD camera (CoolSnap HQ; Photometrics) and deconvolved using the iterative-constrained algorithm (Agard, 1984) and the measured point spread function. One image from the approximate center of z stack is shown in Fig4D 37C pik1-83 panel in J Cell Biol. 187: 967-975. 2009. Images in Fig 4D include CIL#s 13460, 13461, 13462, 13463, 13464, 13465, 13466, 13467."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"Golgi apparatus","onto_id":"GO:0005794"},{"onto_name":"integral to membrane","onto_id":"GO:0016021"},{"onto_name":"vacuolar lumen","onto_id":"GO:0005775"},{"onto_name":"trans-Golgi network","onto_id":"GO:0005802"}],"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"6596","MOLECULARFUNCTION":[{"onto_name":"alpha-1,2-mannosyltransferase activity","onto_id":"GO:0000026"},{"onto_name":"1-phosphatidylinositol 4-kinase activity","onto_id":"GO:0004430"}],"DATAQUALIFICATION":{"free_text":"PROCESSED;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},"DIMENSION":[{"Space":{"Image_size":264,"Pixel_size":{"unit":"microns","value":0.0663},"axis":"X"}},{"Space":{"Image_size":264,"Pixel_size":{"unit":"microns","value":0.0663},"axis":"Y"}},{"Space":{"Image_size":6,"Pixel_size":{"unit":"microns","value":0.4},"axis":"Z"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Saccharomyces cerevisiae S288c","onto_id":"NCBITaxon:559292"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"onto_name":"EGFP","onto_id":"FBbi:00000082"}}},"Citation":{"Title":"Christopher S. Wood, Karl R. Schmitz, Nicholas J. Bessman, Thanuja Gangi Setty, Kathryn M. Ferguson, Christopher G. Burd (2011) CIL:13465, Saccharomyces cerevisiae S288c. CIL. Dataset","ARK":"ark:\/b7295\/w9cil13465","DOI":"doi:10.7295\/W9CIL13465"}}}