{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1280980800},"Data_type":{"Still_image":false,"Z_stack":true,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"728.zip","Size":138669883,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"728.jpg","Size":397930,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"728.tif","Size":135800000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"Contributors":["George H. Patterson"]},"BIOLOGICALPROCESS":{"onto_name":"plasma membrane organization","onto_id":"GO:0007009"},"CELLLINE":{"onto_name":"COS-7","onto_id":"MCC:0000112"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"COS 7 cell expressing Organelle LightsTM PM-GFP (Invitrogen)consisting of the myristoylation\/palmitoylation sequence from Lck\ntyrosine kinase.  Images were collected with a 63X 1.4 NA Plan-Apochromat oil objective on a Zeiss LSM510 META (Carl Zeiss,Thornwood, NY) using 488 nm excitation, HFT 488 dichroic, and LP505 emission filter.  Image size is 1024 x 1024, pixel size is 0.1 µm x 0.1 µm and pixel time was set to 1.6 µsec.  Detector pinhole was set to collect 29 ~0.5 µm slices at 0.5 µm intervals"},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":{"onto_name":"plasma membrane","onto_id":"GO:0005886"},"RELATIONTOINTACTCELL":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"SOURCEOFCONTRAST":{"onto_name":"EGFP","onto_id":"FBbi:00000082"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"single-spot confocal microscopy","onto_id":"FBbi:00000252"},"DIMENSION":[{"Space":{"Image_size":1024,"Pixel_size":{"unit":"microns","value":0.0952},"axis":"X"}},{"Space":{"Image_size":1024,"Pixel_size":{"unit":"microns","value":0.0952},"axis":"Y"}},{"Space":{"Image_size":64,"Pixel_size":{"unit":"microns","value":0.5},"axis":"Z"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Chlorocebus sabaeus","onto_id":"NCBITaxon:60711"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"onto_name":"UV\/visible\/IR photons","onto_id":"FBbi:00000378"}}},"Citation":{"Title":"George H. Patterson (2010) CIL:728, Chlorocebus sabaeus. CIL. Dataset","ARK":"ark:\/b7295\/w9cil728","DOI":"doi:10.7295\/W9CIL728"}}}