{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1306468800},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"13601.jpg","Size":210906,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"13601.zip","Size":4352719,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"13601.tif","Size":4400000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":["JCB 189: 1157-1169, 2010"],"Contributors":["Yuting Sun","Yejing Ge","Jenny Drnevich","Yong Zhao","Mark Band","Jie Chen"],"PUBMED":["20566686"]},"BIOLOGICALPROCESS":{"onto_name":"myoblast fusion","onto_id":"GO:0007520"},"PROCESSINGHISTORY":{"free_text":"brightness and contrast adjusted"},"CELLLINE":{"onto_name":"C2C12","onto_id":"MCC:0000079"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"The C2C12 cell line, a mouse myoblast line, was used here to study the regulatory factors in myogenic differentiation. After cultured in differentiation medium for 3 days, these cells differentiated into myotubes (Green) containing multiple nuclei (Blue). The C2C12 cells stably expressing FLAG-tagged rapamycin-resistant, kinase-inactive (RR\/KI) mTOR were induced to differentiate in the presence of 50nM rapamycin (Rap) for 3 days. 0.2μg\/ml follistatin was added to the medium for the last 2 days of differentiation. Follistatin was able to rescue the effect of Rap to allow myotube maturation.\n\nThis image is one of different treatments in Figure 9B from JCB 189: 1157-1169, 2010. See also CIL: 13592, 13593, 13594."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"nucleus","onto_id":"GO:0005634"},{"onto_name":"muscle myosin complex","onto_id":"GO:0005859"}],"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":[{"onto_name":"compartmentalization of stain or label","onto_id":"FBbi:00000604"},{"onto_name":"distribution of epitope","onto_id":"FBbi:00000592"}],"GROUP_ID":"8288","MOLECULARFUNCTION":{"free_text":"mTOR inhibition"},"TECHNICALDETAILS":{"free_text":"The image was taken by leica DMI 4000B microscope equipped with a QImaging RETIGA Exi camera and Leica 10X\/0.22 lens. \n\nMyosin heavy chain (MHC) expressed in myotubes was labeled by MF-20 mouse antibody followed by FITC-anti-mouse IgG secondary antibody. Nuclei were stained by DAPI. MHC and nuclei were pseudocolored in green and blue, respectively."},"DATAQUALIFICATION":{"free_text":"PROCESSED;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},"DIMENSION":[{"Space":{"Image_size":1392,"Pixel_size":{"unit":"microns","value":0.645},"axis":"X"}},{"Space":{"Image_size":1040,"Pixel_size":{"unit":"microns","value":0.645},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"},"PREPARATION":[{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},{"onto_name":"permeabilized tissue","onto_id":"FBbi:00000093"}],"VISUALIZATIONMETHODS":[{"onto_name":"Fluorescein (FITC)","onto_id":"FBbi:00000451"},{"onto_name":"4',6-diamidino-2-phenylindole (DAPI)","onto_id":"FBbi:00000056"},{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"}],"CELLTYPE":{"onto_name":"myoblast","onto_id":"CL:0000056"}}},"Citation":{"Title":"Yuting Sun, Yejing Ge, Jenny Drnevich, Yong Zhao, Mark Band, Jie Chen (2011) CIL:13601, Mus musculus, myoblast. CIL. Dataset","ARK":"ark:\/b7295\/w9cil13601","DOI":"doi:10.7295\/W9CIL13601"}}}