{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1320379200},"Data_type":{"Still_image":false,"Z_stack":false,"Video":true,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"28781.jpg","Size":21527,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"28781.zip","Size":1110144,"Mime_type":"application\/zip"},{"File_type":"Mp4","File_path":"28781_web.mp4","Size":527778,"Mime_type":"video\/mp4"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":["J Cell Biol. 2007 Mar 12;176(6):757-63. Epub 2007 Mar 5."],"Contributors":["Paul S. Maddox","Francie Hyndman","Joost Monen","Karen Oegema","Arshad Desai"],"PUBMED":["17339379"]},"BIOLOGICALPROCESS":[{"free_text":"KNL-2 depletion"},{"onto_name":"mitosis","onto_id":"GO:0007067"},{"onto_name":"prophase","onto_id":"GO:0051324"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":[{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},{"onto_name":"optical path length gradient","onto_id":"FBbi:00000312"}],"IMAGEDESCRIPTION":{"free_text":"Spinning disk confocal time-lapse imaging of the first mitosis in a C. elegans embryo expressing GFP-CeCENP-A in which KNL-2 has been depleted by RNAi. GFP-CeCENP-A was imaged (strain OD101). Diffuse nuclear GFP signal is evident during prophase. However, unlike the control (CIL 28780), no discrete GFP foci were seen at any point. The video is ∼12 min long, and the frame is ∼30 µm top to bottom. DIC is shown on the left, and GFP is shown on the right."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":{"free_text":"CeCNP-A"},"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":[{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},{"onto_name":"boundaries between regions with different refractive index","onto_id":"FBbi:00000599"}],"GROUP_ID":"11164","TECHNICALDETAILS":{"free_text":"Images were acquired via a spinning disk confocal microscope (CSU10; McBain Instruments) mounted on an inverted microscope (TE2000e; Nikon). Strain TH32 coexpressing GFP-histone H2b and GFP\u2013γ-tubulin was imaged using a 60× 1.4 NA plan Apo objective with 1.5× auxiliary magnification and a cooled CCD camera (Orca ER; Hamamatsu) binning 2 × 2.  Strain OD31 expressing GFP\u2013KNL-2 was imaged in the same manner without the 1.5× auxiliary magnification."},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":[{"onto_name":"spinning disk confocal microscopy","onto_id":"FBbi:00000253"},{"onto_name":"differential interference contrast microscopy","onto_id":"FBbi:00000245"}],"DIMENSION":[{"Space":{"Image_size":520,"axis":"X"}},{"Space":{"Image_size":150,"axis":"Y"}},{"Time":{"unit":"seconds","value":10,"frame":"73"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Caenorhabditis elegans","onto_id":"NCBITaxon:6239"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":[{"onto_name":"EGFP","onto_id":"FBbi:00000082"},{"onto_name":"visualization of contiguous regions","onto_id":"FBbi:00000396"}],"CELLTYPE":{"free_text":"embryo"}}},"Citation":{"Title":"Paul S. Maddox, Francie Hyndman, Joost Monen, Karen Oegema, Arshad Desai (2011) CIL:28781, Caenorhabditis elegans, embryo. CIL. Dataset","ARK":"ark:\/b7295\/w9cil28781","DOI":"doi:10.7295\/W9CIL28781"}}}