{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1291093200},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"350.zip","Size":3419622,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"350.tif","Size":3500000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"350.jpg","Size":495435,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"Contributors":["Hanna Osinska"],"PUBMED":["18345011"]},"BIOLOGICALPROCESS":{"onto_name":"plasma membrane organization","onto_id":"GO:0007009"},"PROCESSINGHISTORY":{"free_text":"unprocessed raw data"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"Thin section electron microscopy of gastrocnemius (skeletal) muscle from a wild type mouse.  Section was stained with warm uranyl acetate stain.\n\nSample was viewed with a Hitachi 7600 electron microscope (accelerating voltage 80 KV) and imaged with an AMT digital camera.   Image taken at 40,000X magnification.\n\nThis image is part of a set of wild type gastrocnemius muscle images that served as controls for the study of the delta-sarcoglycan knockout mouse.  Both the set of wild-type and delta-sarcoglycan knockout mouse images are part of The CELL:  an image library.\n\nReference:  Nat Med. 2008 Apr;14(4):442-7.  \nGenetic and pharmacologic inhibition of mitochondrial-dependent necrosis attenuates muscular dystrophy.\nMillay DP, Sargent MA, Osinska H, Baines CP, Barton ER, Vuagniaux G, Sweeney HL, Robbins J, Molkentin JD  (tissue preparation described in Fewell JG, et al. A treadmill exercise regimen for identifying cardiovascular phenotypes in transgenic mice. Am J Physiol. 1997;273:H1595\u2013H1605)"},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":{"onto_name":"dystroglycan complex","onto_id":"GO:0016011"},"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":[{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"}],"GROUP_ID":"2306","MOLECULARFUNCTION":{"onto_name":"structural molecule activity","onto_id":"GO:0005198"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},"DIMENSION":[{"Space":{"Image_size":1696,"Pixel_size":{"unit":"nanometers","value":2.32},"axis":"X"}},{"Space":{"Image_size":2016,"Pixel_size":{"unit":"nanometers","value":2.32},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"osmium tetroxide fixed tissue","onto_id":"FBbi:00000012"}],"VISUALIZATIONMETHODS":{"onto_name":"detection of electrons","onto_id":"FBbi:00000375"},"CELLTYPE":{"onto_name":"skeletal muscle cell","onto_id":"CL:0000188"}}},"Citation":{"Title":"Hanna Osinska (2010) CIL:350, Mus musculus, skeletal muscle cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil350","DOI":"doi:10.7295\/W9CIL350"}}}