{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1300161600},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"20814.zip","Size":10268598,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"20814.tif","Size":5200000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"20814.jpg","Size":1879359,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":["J. Cell Sci. 112:3733-3745, 1999"],"Contributors":["Richard Allen (University of Hawaii)"],"PUBMED":["10523509"]},"BIOLOGICALPROCESS":[{"onto_name":"contractile vacuole organization","onto_id":"GO:0033298"},{"onto_name":"cytoplasmic microtubule organization","onto_id":"GO:0031122"}],"PROCESSINGHISTORY":[{"onto_name":"recorded image","onto_id":"FBbi:00000265"},{"onto_name":"film","onto_id":"FBbi:00000303"},{"free_text":"Print from negative scanned for Photoshop."}],"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"Cubic membrane such as this array represents the cell\u2019s method of storing large quantities of lipid and protein bilayers into the smallest possible volume. These membranes can quickly expand into planar sheets when the ampulla or the collecting canal needs to expand. These arrays actually bury the microtubular ribbons between the membrane of the collecting canal and these three-dimensional arrays. TEM taken on 8\/8\/96 by R. Allen with Zeiss 10A operating at 80kV. Neg. 31,500X. Bar = 0.2µm. Adapted with permission from the J. Cell Sci. 112:3733-3745, 1999. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:20814) is in the library and available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http:\/\/www5.pbrc.hawaii.edu\/allen\/)."},"ITEMTYPE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"}],"CELLULARCOMPONENT":[{"onto_name":"contractile vacuole","onto_id":"GO:0000331"},{"onto_name":"cytoplasmic microtubule","onto_id":"GO:0005881"},{"onto_name":"contractile vacuolar membrane","onto_id":"GO:0031164"},{"onto_name":"intrinsic to contractile vacuolar membrane","onto_id":"GO:0031311"}],"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},"GROUP_ID":"5662","DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":[{"onto_name":"detection of electrons","onto_id":"FBbi:00000375"},{"onto_name":"film","onto_id":"FBbi:00000303"}],"DIMENSION":[{"Space":{"Image_size":2015,"axis":"X"}},{"Space":{"Image_size":2550,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Paramecium multimicronucleatum","onto_id":"NCBITaxon:44030"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"osmium tetroxide fixed tissue","onto_id":"FBbi:00000012"},{"onto_name":"tissue in epoxy resin embedment","onto_id":"FBbi:00000018"},{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"}],"VISUALIZATIONMETHODS":[{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},{"onto_name":"osmium tetroxide","onto_id":"FBbi:00000571"},{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"}],"CELLTYPE":[{"onto_name":"cell by organism","onto_id":"CL:0000004"},{"onto_name":"eukaryotic cell","onto_id":"CL:0000255"},{"free_text":"Eukaryotic Protist"},{"free_text":"Ciliated Protist"}]}},"Citation":{"Title":"Richard Allen (University of Hawaii) (2011) CIL:20814, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset","ARK":"ark:\/b7295\/w9cil20814","DOI":"doi:10.7295\/W9CIL20814"}}}