{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1291179600},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"10008.zip","Size":23050754,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"10008.jpg","Size":3801859,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"10008.tif","Size":11400000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Href":"http:\/\/www5.pbrc.hawaii.edu\/allen\/"}],"Contributors":["Richard Allen"]},"BIOLOGICALPROCESS":[{"onto_name":"cytoplasm organization","onto_id":"GO:0007028"},{"onto_name":"organelle organization","onto_id":"GO:0006996"},{"onto_name":"organelle localization","onto_id":"GO:0051640"},{"free_text":"organelle development"}],"PROCESSINGHISTORY":[{"onto_name":"film","onto_id":"FBbi:00000303"},{"free_text":"Print from negative scanned for Photoshop."}],"CELLLINE":{"free_text":"Carolina Biological Supply Company, NC, U.S.A."},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"Toxicysts in the cytoplasm of cross sectioned non-dividing Didinium. Toxicysts (extrusomes) are multilayered cylinders that are discharged from the cytopharynx region during capture of prey such as Paramecium. Lipid is stored as large non-membrane enclosed dense bodies in the cytosol. TEM taken on 2\/18\/69 by R. Allen with Philips 300. Neg. 20,500X. Bar = 0.5µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. A raw, unprocessed, high resolution version of this image (CIL:9929) is in the library and available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http:\/\/www5.pbrc.hawaii.edu\/allen\/)."},"ITEMTYPE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"}],"CELLULARCOMPONENT":[{"onto_name":"rough endoplasmic reticulum","onto_id":"GO:0005791"},{"onto_name":"lipid particle","onto_id":"GO:0005811"},{"free_text":"extrusome"},{"free_text":"toxicyst"}],"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},"GROUP_ID":"3411","TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":[{"onto_name":"detection of electrons","onto_id":"FBbi:00000375"},{"onto_name":"film","onto_id":"FBbi:00000303"}],"DIMENSION":[{"Space":{"Image_size":3732,"axis":"X"}},{"Space":{"Image_size":3032,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Didinium nasutum","onto_id":"NCBITaxon:5997"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"osmium tetroxide fixed tissue","onto_id":"FBbi:00000012"},{"onto_name":"tissue in epoxy resin embedment","onto_id":"FBbi:00000018"},{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"}],"VISUALIZATIONMETHODS":[{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},{"onto_name":"osmium tetroxide","onto_id":"FBbi:00000571"},{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"}],"CELLTYPE":[{"onto_name":"eukaryotic cell","onto_id":"CL:0000255"},{"free_text":"Eukaryotic Protist"},{"free_text":"Ciliated Protist"}]}},"Citation":{"Title":"Richard Allen (2010) CIL:10008, Didinium nasutum, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset","ARK":"ark:\/b7295\/w9cil10008","DOI":"doi:10.7295\/W9CIL10008"}}}