{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1306123200},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"26551.zip","Size":529144,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"26551.tif","Size":600000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"26551.jpg","Size":89680,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Href":"http:\/\/jcb-dataviewer.rupress.org\/jcb\/browse\/3112\/"}],"PUBLISHED":["J. Cell Biol. 2010. Vol. 190(4):663\u2013674"],"Contributors":["Chan-Soo Lee","Chang-Ki Choi","Eun-Young Shin","Martin Alexander Schwartz","Eung-Gook Kim"],"PUBMED":["PMID:20713598"]},"BIOLOGICALPROCESS":{"onto_name":"regulation of cell migration","onto_id":"GO:0030334"},"CELLLINE":{"onto_name":"3T3-Swiss albino","onto_id":"MCC:0000012"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"To study the molecular mechanism by which nonmuscle myosin II (MII) regulates protrusion and adhesion dynamics in migrating cells, Swiss3T3 cells were co-stained for TRIO (green) and myosin IIA (red). These findings help elucidate a functional link between MII and Rac1\/Cdc42 GTPases, which may regulate protrusion\/adhesion dynamics in migrating cells.  This image is the original data file from Fig. 2F \u201Ccolocalization of MII with GEFs\u201D, J. Cell Biol. 2010. Vol. 190(4):663\u2013674."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"lamellipodium","onto_id":"GO:0030027"},{"onto_name":"myosin II complex","onto_id":"GO:0016460"},{"onto_name":"actin filament","onto_id":"GO:0005884"}],"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"distribution of epitope","onto_id":"FBbi:00000592"},"GROUP_ID":"8283","TECHNICALDETAILS":{"free_text":"Cells were cultured in DME (Invitrogen) supplemented with 10% fetal bovine serum and 100 U\/ml penicillin\/streptomycin (Invitrogen) at 37°C in a humidified 5% CO2 incubator. Cells were serum-starved overnight, then fixed using 3.7% paraformaldehyde in PBS for 15 min, permeabilized using 0.2% Triton X-100 in PBS for 2 min, blocked with 2% BSA in PBS, and stained with the indicated primary antibodies at 4°C overnight, followed by incubation with a secondary Alexa Fluor 488\u2013, 546\u2013 or 594\u2013conjugated antibody. Images were captured by Leica TCS SP2 confocal microscope with HCX PL APO 63X objective."},"DATAQUALIFICATION":{"free_text":"RAW"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"confocal microscopy","onto_id":"FBbi:00000251"},"DIMENSION":[{"Space":{"Image_size":512,"axis":"X"}},{"Space":{"Image_size":512,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"},"PREPARATION":[{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},{"onto_name":"detergent permeabilized","onto_id":"FBbi:00000262"}],"VISUALIZATIONMETHODS":[{"onto_name":"Alexa Fluor 488","onto_id":"FBbi:00000440"},{"onto_name":"Alexa Fluor 546","onto_id":"FBbi:00000441"}],"CELLTYPE":{"onto_name":"fibroblast","onto_id":"CL:0000057"}}},"Citation":{"Title":"Chan-Soo Lee, Chang-Ki Choi, Eun-Young Shin, Martin Alexander Schwartz, Eung-Gook Kim (2011) CIL:26551, Mus musculus, fibroblast. CIL. Dataset","ARK":"ark:\/b7295\/w9cil26551","DOI":"doi:10.7295\/W9CIL26551"}}}