{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1307073600},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"24794.jpg","Size":102491,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"24794.tif","Size":2200000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"24794.zip","Size":2204402,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":["J Cell Biol. 1999 May 31;145(5):1009-26."],"Contributors":["Tatyana M. Svitkina","Gary G. Borisy"],"PUBMED":["10352018"]},"BIOLOGICALPROCESS":{"onto_name":"cellular macromolecule localization","onto_id":"GO:0070727"},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Localization of Arp2\/3 complex in the lamellioidia of a Xenopus keratocytes. Staining with p21 antibody (green) and TRIT-phalloidin (red) shows Arp2\/3 complex highly enriched in lamellipodia.  CIL 24795 shows an immuno EM image of Arp2\/3 localization in a Xenopus keratocyte.  CIL 24796 and  24797 show corresponding images in fibroblasts.  Image corresponds to Figure 3a-c from J Cell Biol. 1999 May 31;145(5):1009-26."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"lamellipodium","onto_id":"GO:0030027"},{"onto_name":"actin cytoskeleton","onto_id":"GO:0015629"}],"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"9875","TECHNICALDETAILS":{"free_text":"Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998)."},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},"DIMENSION":[{"Space":{"Image_size":699,"Pixel_size":{"unit":"microns","value":0.126},"axis":"X"}},{"Space":{"Image_size":1044,"Pixel_size":{"unit":"microns","value":0.126},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Xenopus laevis","onto_id":"NCBITaxon:8355"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"methanol fixed tissue","onto_id":"FBbi:00000007"}],"VISUALIZATIONMETHODS":[{"onto_name":"Fluorescein (FITC)","onto_id":"FBbi:00000451"},{"onto_name":"Tetramethyl rhodamine (TRITC)","onto_id":"FBbi:00000454"},{"onto_name":"phalloidin","onto_id":"FBbi:00000100"},{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"}],"CELLTYPE":{"free_text":"keratocyte"}}},"Citation":{"Title":"Tatyana M. Svitkina, Gary G. Borisy (2011) CIL:24794, Xenopus laevis, keratocyte. CIL. Dataset","ARK":"ark:\/b7295\/w9cil24794","DOI":"doi:10.7295\/W9CIL24794"}}}