{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1364875200},"Data_type":{"Still_image":false,"Z_stack":true,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"44501.zip","Size":62922311,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"44501.jpg","Size":637464,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"44501.tif","Size":63000000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"OTHER":["Contact: assafzar@gmail.com"],"Contributors":["Assaf Zaritsky","Doron Kaplan"]},"BIOLOGICALPROCESS":[{"onto_name":"wound healing, spreading of epidermal cells","onto_id":"GO:0035313"},{"free_text":"cell migration"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":{"onto_name":"MDCK-II","onto_id":"MCC:0000321"},"PARAMETERIMAGED":{"onto_name":"optical path length gradient","onto_id":"FBbi:00000312"},"IMAGEDESCRIPTION":{"free_text":"Time series light microscopy images illustrating a wound healing assay. A monolayer of MDCK (Madin-Darby Canine Kidney) epithelial cells is scratched to create a 'wound' about 300 micrometers in width, after which the growth of the cells to fill the wound is monitored by recording images over the course of 15h. CIL:44511 contains a movie of the process. This image is part of a group of 12 that includes control cells (CIL:44501, 502, 506, 507, 508) and after addition of  Hepatocyte Growth Factor \/Scatter Factor (HGF\/SF) to activate HGF\/SF-Met signaling (CIL:44503, 504, 505, 509, 510).  Also in the group are two movies, one control (CIL:44511), and one HGF\/SF treated (CIL:44512). For  additional wound healing assays see CIL:43401 and images grouped with it."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"boundaries between regions with different refractive index","onto_id":"FBbi:00000599"},"GROUP_ID":"14702","TECHNICALDETAILS":{"free_text":"Madin-Darby Canine Kidney (MDCK) epithelial cells expressing YFP-membrane were maintained in DMEM supplemented with 5% fetal FCS in a 37°C, 5% CO2 incubator.\nWound healing assay: Cells were grown to 90% confluence in 24-well plates. Prior to scratching, the cells were starved by changing the medium to DMEM plus 0.1% FCS (starvation medium) for 24 hours. The medium was then changed to either fresh starvation medium (control), or starvation medium with 80 ng ml-1 HGF\/SF for an additional 2 hours. A scratch of approximately 300 μm in width was generated using a 200 μl tip. The plate was subjected to time lapse microscopy in a stage incubator (OKOLAB, Italy) on a computer-controlled motorized stage of a confocal microscope (CLSM-510, Carl Zeiss, Germany), used in non-confocal DIC mode, with a 10x (NA 0.30) objective. Image acquisition was initiated 2 hours post scratching. Images were acquired every 15.7 minutes for 15 hours. The coordinates of each scratch were predefined, and a macro that repetitively positions the field of view at each point was executed. The acquired differential interference contrast (DIC) channel of the time-lapse sequence was used for the analysis."},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"differential interference contrast microscopy","onto_id":"FBbi:00000245"},"DIMENSION":[{"Space":{"Image_size":1024,"Pixel_size":{"unit":"microns","value":0.879},"axis":"X"}},{"Space":{"Image_size":1024,"Pixel_size":{"unit":"microns","value":0.879},"axis":"Y"}},{"Time":{"unit":"seconds","value":942,"frame":"60"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Canis lupus familiaris","onto_id":"NCBITaxon:9615"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"onto_name":"visualization of contiguous regions","onto_id":"FBbi:00000396"},"CELLTYPE":{"onto_name":"kidney epithelial cell","onto_id":"CL:0002518"}}},"Citation":{"Title":"Assaf Zaritsky, Doron Kaplan (2013) CIL:44501, Canis lupus familiaris, kidney epithelial cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil44501","DOI":"doi:10.7295\/W9CIL44501"}}}