{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1409976000},"Data_type":{"Still_image":false,"Z_stack":false,"Video":true,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"47580.zip","Size":1368192,"Mime_type":"application\/zip"},{"File_type":"Mp4","File_path":"47580_web.mp4","Size":204350,"Mime_type":"video\/mp4"},{"File_type":"Jpeg","File_path":"47580.jpg","Size":30730,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"Contributors":["Mark Harding"]},"BIOLOGICALPROCESS":{"free_text":"immune cell recruitment"},"PROCESSINGHISTORY":{"free_text":"Converted from volocity to .avi"},"IMAGEDESCRIPTION":{"free_text":"Visualization of neutrophil recruitment in capillaries proximal to agarose beads. (Figure 3 - Mac1)"},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"SOURCEOFCONTRAST":{"free_text":"antibody labelling"},"RELATIONTOINTACTCELL":{"free_text":"intact cells-in vivo imaging"},"GROUP_ID":"16608","MOLECULARFUNCTION":{"free_text":"blocking of integrin Mac1"},"TECHNICALDETAILS":{"free_text":"Mice were anesthetized with ketamine\/xylazine, and then subcutaneous tissue on a dorsal flap was exteriorized.  GFP staphylococcus aureus beads were inserted.  Antibodies were injected into the vasculature to label neutrophils (red) and endothelial cells (blue).  Blocking antibodies against the molecule Mac1 were administered after 2 hours of infection."},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"spinning disk confocal microscopy","onto_id":"FBbi:00000253"},"DIMENSION":[{"Space":{"Pixel_size":{"unit":"microns","value":512},"axis":"X"}},{"Space":{"Pixel_size":{"unit":"microns","value":512},"axis":"Y"}}],"SPECIESTAXASPECIFIC":{"free_text":":laboratory mouse (C57 Black 6); Staphylococcus aureus (USA300 strain)"},"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Staphylococcus aureus","onto_id":"NCBITaxon:1280"},"CELLTYPE":[{"onto_name":"neutrophil","onto_id":"CL:0000775"},{"onto_name":"endothelial cell","onto_id":"CL:0000115"}],"PATHOLOGICALPROCESS":{"free_text":":Infection of subcutaneous tissue with staphylococcus aureus bead"}}},"Citation":{"Title":"Mark Harding (2014) CIL:47580, Staphylococcus aureus, neutrophil, endothelial cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil47580","DOI":"doi:10.7295\/W9CIL47580"}}}