{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1314676800},"Data_type":{"Still_image":false,"Z_stack":true,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"36969.tif","Size":17400000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"36969.jpg","Size":6395,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"36969.zip","Size":17402252,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"Contributors":["Jennifer Bestman","Holly Cline"]},"BIOLOGICALPROCESS":[{"onto_name":"neuron development","onto_id":"GO:0048666"},{"onto_name":"morphogenesis of a branching structure","onto_id":"GO:0001763"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"This is IMAGE #2 (day 2) of a time lapse series contained with in this image group. It is a neuron from the optic tectum of an albino Xenopus laevis tadpole CNS, stage 47. \n\nThe images were acquired daily over 3 days and show dendritic arbor development and morphological plasticity of the dendritic arbor with branch additions and retractions.\n\nThe cell was transfected with in vivo electroporation of plasmid DNA encoding eYFP ~24 hours before the first image was acquired. The intact, living tadpole was anesthetized, positioned under a glass coverslip and this image was taken directly through its transparent skin using a custom built two-photon microscope."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"dendrite","onto_id":"GO:0030425"},{"onto_name":"cytoplasm","onto_id":"GO:0005737"},{"onto_name":"axon","onto_id":"GO:0030424"},{"onto_name":"neuronal cell body","onto_id":"GO:0043025"}],"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"9186","TECHNICALDETAILS":{"free_text":"Specifications of the custom built two-photon microscope, including the laser sources, signal amplification, PMT specifications, and YFP filter sets are described in: \nRuthazer ES, Li J, Cline HT. 2006. Stabilization of axon branch dynamics by synaptic maturation. J Neurosci 26(13):3594-3603. \n\nThe methods of cell transfection with electroporation can be found here: \nBestman JE, Ewald RC, Chiu S-L, Cline HT. 2006. In vivo single-cell electroporation for transfer of DNA and macromolecules. Nat Protocols 1(3):1267-1272. \n\nFurther explanation can be found here:\nBestman JE, Cline HT. 2008. The RNA binding protein CPEB regulates dendrite morphogenesis and neuronal circuit assembly in vivo. Proc Natl Acad Sci U S A 105(51):20494-20499.\n\nBestman JE, Cline HT. 2009. The Relationship between Dendritic Branch Dynamics and CPEB-Labeled RNP Granules Captured in Vivo. Front Neural Circuits 3:10."},"DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"multi-photon microscopy","onto_id":"FBbi:00000255"},"DIMENSION":[{"Space":{"Image_size":512,"Pixel_size":{"unit":"microns","value":0.233},"axis":"X"}},{"Space":{"Image_size":512,"Pixel_size":{"unit":"microns","value":0.233},"axis":"Y"}},{"Space":{"Image_size":33,"Pixel_size":{"unit":"microns","value":1.5},"axis":"Z"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Xenopus laevis","onto_id":"NCBITaxon:8355"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"onto_name":"EYFP","onto_id":"FBbi:00000084"},"CELLTYPE":{"onto_name":"CNS neuron (sensu Vertebrata)","onto_id":"CL:0000117"}}},"Citation":{"Title":"Jennifer Bestman, Holly Cline (2011) CIL:36969, Xenopus laevis, CNS neuron (sensu Vertebrata). CIL. Dataset","ARK":"ark:\/b7295\/w9cil36969","DOI":"doi:10.7295\/W9CIL36969"}}}