{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1299560400},"Data_type":{"Still_image":false,"Z_stack":true,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"10469.tif","Size":25200000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"10469.jpg","Size":78932,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"10469.zip","Size":25183450,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"Contributors":["John Murray (University of Pennsylvania)"]},"BIOLOGICALPROCESS":[{"onto_name":"regulation of cell shape","onto_id":"GO:0008360"},{"onto_name":"microtubule cytoskeleton organization","onto_id":"GO:0000226"}],"PROCESSINGHISTORY":{"onto_name":"3D-deconvolution","onto_id":"FBbi:00000357"},"CELLLINE":{"free_text":"Toxoplasma gondii RH stable cell line"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing EGFP-tagged Tgbeta3-tubulin.  Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 60X water immersion, 1.2 NA. Filters: GFP, ex 450-490 em 500-540. Captured with CoolSnapHQ\/ICX285. 3D-Deconvolved stack corresponds to raw data stack: CIL# 10468. Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details."},"ITEMTYPE":[{"onto_name":"recorded image","onto_id":"FBbi:00000265"},{"onto_name":"charge coupled device (CCD)","onto_id":"FBbi:00000294"}],"CELLULARCOMPONENT":{"onto_name":"cortical microtubule","onto_id":"GO:0055028"},"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"7515","MOLECULARFUNCTION":{"onto_name":"structural constituent of cytoskeleton","onto_id":"GO:0005200"},"HUMAN_DISEASE":[{"onto_name":"toxoplasmosis","onto_id":"DOID:9965"}],"DATAQUALIFICATION":{"free_text":"PROCESSED;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},"DIMENSION":[{"Space":{"Image_size":1024,"Pixel_size":{"unit":"microns","value":0.1067},"axis":"X"}},{"Space":{"Image_size":1024,"Pixel_size":{"unit":"microns","value":0.1067},"axis":"Y"}},{"Space":{"Image_size":12,"Pixel_size":{"unit":"microns","value":0.3},"axis":"Z"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Toxoplasma gondii RH","onto_id":"NCBITaxon:383379"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"onto_name":"EGFP","onto_id":"FBbi:00000082"},"PATHOLOGICALPROCESS":[{"onto_id":"DOID:DOID:9965"},{"onto_id":"IDO:IDO:0000038"}]}},"Citation":{"Title":"John Murray (University of Pennsylvania) (2011) CIL:10469, Toxoplasma gondii RH. CIL. Dataset","ARK":"ark:\/b7295\/w9cil10469","DOI":"doi:10.7295\/W9CIL10469"}}}