{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1280980800},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"715.tif","Size":32300000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"715.zip","Size":32432134,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"715.jpg","Size":6251694,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"Contributors":["Woodcock, Chris"],"PUBMED":["1757505"]},"BIOLOGICALPROCESS":{"onto_name":"chromatin assembly","onto_id":"GO:0031497"},"PROCESSINGHISTORY":{"free_text":"raw unprocessed data"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"X-ray analysis shows that the nucleated erythrocytes of the amphibian Necturus maculosus (Mud Puppy) contains more ordered chromatin than most nuclei.  Here, chromatin fibers were isolated from N. maculosus erythrocyte nuclei using mild micrococcal nuclease digestion, fixed briefly with glutaraldehyde, applied to thin carbon films and negatively stained with methylamine tungstate.  ~30 nm fibers are seen, some exhibiting short regions with apparent structural order."},"ITEMTYPE":{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},"CELLULARCOMPONENT":{"onto_name":"nuclear chromatin","onto_id":"GO:0000790"},"RELATIONTOINTACTCELL":{"onto_name":"isolated subcellular component","onto_id":"FBbi:00000579"},"SOURCEOFCONTRAST":{"onto_name":"elastic scattering","onto_id":"FBbi:00000310"},"MOLECULARFUNCTION":{"free_text":"DNA packaging"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":[{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"},{"onto_name":"detection of electrons","onto_id":"FBbi:00000375"}],"DIMENSION":[{"Space":{"Image_size":4022,"Pixel_size":{"unit":"nanometers","value":0.67},"axis":"X"}},{"Space":{"Image_size":2746,"Pixel_size":{"unit":"nanometers","value":0.67},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Necturus maculosus","onto_id":"NCBITaxon:42757"},"PREPARATION":[{"onto_name":"unembedded tissue","onto_id":"FBbi:00000023"},{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"}],"VISUALIZATIONMETHODS":{"onto_name":"other negative stain","onto_id":"FBbi:00000577"},"CELLTYPE":{"onto_name":"nucleate erythrocyte","onto_id":"CL:0000562"}}},"Citation":{"Title":"Woodcock, Chris (2010) CIL:715, Necturus maculosus, nucleate erythrocyte. CIL. Dataset","ARK":"ark:\/b7295\/w9cil715","DOI":"doi:10.7295\/W9CIL715"}}}