CIL:36755, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist
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- Collection
- Cite This Work
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Allen, Richard (2021). CIL:36755, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0P849TD
- Description
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Paramecium caudatum is a peniculine oligohymenophoran ciliate that has numerous rows of cilia called kineties that run along the posterior to the anterior axis on the cell‚Äôs dorsal side but curve around the oral region on the cell‚Äôs ventral side and end to form the anterior and posterior suture lines that extend from the oral region to the tips of the cell. This is a cross-section of P. caudatum near its anterior end showing a layer of trichocysts that lie under the cell surface. The surface of the cell is sculpted into ridges and the cilia arise from the bases of the concave invaginations. Each concavity represents a section through one kinety so that in this micrograph approximately 58 kineties can be seen to surround the cell near its anterior end. At this low magnification one can also see large numbers of medium-opaque mitochondria near the cell surface and interspersed between the trichocysts. Collidine-buffered fixation. TEM taken on 1/18/74 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 3,750X. Bar = 1µm.
- Date Issued
- 2021
- Researcher
- Methods
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Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:39112) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
- Technical Details
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Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed - Series
- Scientific Name
- Anatomy
- Topics
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 36755
- Related Resource
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL36755
Source data
- License
- Copyright
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Creative Commons Public Domain Dedication
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Constraint(s) on Use: This work may be used without prior permission.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12