{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1321938000},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"39115.zip","Size":1497948,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"39115.tif","Size":1600000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"39115.jpg","Size":448799,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":["KR Miller, GJ Miller, KR McIntyre 1976 The light-harvesting chlrophyll-protein complex of photosystem II"],"Contributors":["Kenneth R Miller","Gayle J Miller","Katherine R McIntyre"],"PUBMED":["993264"]},"BIOLOGICALPROCESS":{"onto_name":"C4 photosynthesis","onto_id":"GO:0009760"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"Freeze-fracture image of a chloroplast from barley (Hordeum vulgare) illustrating the characteristic size and distribution of particles on the EFu and EFs thylakoid membrane faces. The inset gives a higher magnification view of the particles on the stacked ES face. Other images in this group show the changes in particle distribution in a pale green mutant that lacks chlorophyll b."},"ITEMTYPE":{"onto_name":"film","onto_id":"FBbi:00000303"},"CELLULARCOMPONENT":[{"onto_name":"chloroplast","onto_id":"GO:0009507"},{"onto_name":"plastid thylakoid","onto_id":"GO:0031976"},{"onto_name":"photosystem II","onto_id":"GO:0009523"}],"RELATIONTOINTACTCELL":{"onto_name":"isolated subcellular component","onto_id":"FBbi:00000579"},"SOURCEOFCONTRAST":{"onto_name":"differences in deposition of metal shadow","onto_id":"FBbi:00000601"},"GROUP_ID":"9897","TECHNICALDETAILS":{"free_text":"Wildtype and mutant plants were were grown under a 16-hr day regime, harvested at 3 weeks, and chloroplasts isolated. For freeze fracture, isolated chloroplasts were made 30% in glycerol, plunge frozen, and cleaved, etched, and shadowed in a Balzers freeze-fracture unit. Replicas were examined with a Philips 300 transmission EM and images recorded on film. See Fig 11 in KR Miller et al. 1976 J. Cell Biol 71, 624-638."},"DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},"DIMENSION":[{"Space":{"Image_size":1248,"axis":"X"}},{"Space":{"Image_size":1200,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Hordeum vulgare subsp. vulgare","onto_id":"NCBITaxon:112509"},"PREPARATION":{"onto_name":"freeze_fracture\/freeze_etch","onto_id":"FBbi:00000419"},"VISUALIZATIONMETHODS":{"onto_name":"shadowing and plating","onto_id":"FBbi:00000398"},"CELLTYPE":{"free_text":"leaf"}}},"Citation":{"Title":"Kenneth R Miller, Gayle J Miller, Katherine R McIntyre (2011) CIL:39115, Hordeum vulgare subsp. vulgare, leaf. CIL. Dataset","ARK":"ark:\/b7295\/w9cil39115","DOI":"doi:10.7295\/W9CIL39115"}}}