Miller, Kenneth R.; Miller, Gayle J.; McIntyre, Katherine R. (2021). CIL:39115, Hordeum vulgare subsp. vulgare, leaf. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0862F3G

Freeze-fracture image of a chloroplast from barley (Hordeum vulgare) illustrating the characteristic size and distribution of particles on the EFu and EFs thylakoid membrane faces. The inset gives a higher magnification view of the particles on the stacked ES face. Other images in this group show the changes in particle distribution in a pale green mutant that lacks chlorophyll b.

• 2021

• McIntyre, Katherine R.
• Miller, Gayle J.
• Miller, Kenneth R.

Wildtype and mutant plants were were grown under a 16-hr day regime, harvested at 3 weeks, and chloroplasts isolated. For freeze fracture, isolated chloroplasts were made 30% in glycerol, plunge frozen, and cleaved, etched, and shadowed in a Balzers freeze-fracture unit. Replicas were examined with a Philips 300 transmission EM and images recorded on film. See Fig 11 in KR Miller et al. 1976 J. Cell Biol 71, 624-638.

Preparation: freeze_fracture/freeze_etch
Relation to intact cell: isolated subcellular component
Item type: film
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Data qualification: Processed

• Cell Image Library Group ID: 9897

• Hordeum vulgare subsp. vulgare

• Chloroplast
• Leaf
• Photosystem II
• Plastid thylakoid

• C4 photosynthesis

• data
• image

• No linguistic content; Not applicable

Samplenumber: 39115

Doi: https://doi.org/10.6075/J0862F3G

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL39115

Reference

• KR Miller, GJ Miller, KR McIntyre 1976 The light-harvesting chlrophyll-protein complex of photosystem II https://www.ncbi.nlm.nih.gov/pubmed/?term=993264

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2022-08-12